SARS-CoV-2 pseudovirus enters the host cells through spike protein-CD147 in an Arf6-dependent mannerSARS-CoV-2 슈도바이러스는 Arf6 의존적 방식으로 스파이크 단백질-CD147을 통해 숙주 세포에 들어갑니다.Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 approach Arf6 blockade CD147 cells clathrin Clathrin-mediated endocytosis clinically coronavirus endocytic endocytic mechanism endocytosis evaluate feasible function host cell host cells Huh-7 cell Huh-7 cells independent independent of indicated infected Vero E6 cells Infection inhibited inhibitor knockdown Localization macropinocytosis pathway Prevent pseudovirus pseudovirus infection public health RAB5A SARS-CoV-2 SARS-CoV-2 entry SARS-COV-2 infection SARS-CoV-2 pseudovirus SARS-CoV-2 pseudovirus infection severe acute respiratory syndrome Coronavirus Spike protein spike protein. Spread the SARS-CoV-2 variant Vero E6 VERO E6 cells viral infection weakened [DOI] 10.1080/22221751.2022.2059403 PMC 바로가기 [Article Type] Article
Modulating Fingolimod (FTY720) Anti-SARS-CoV-2 Activity Using a PLGA-Based Drug Delivery SystemArticle Published on 2022-07-182022-10-04 Journal: ACS applied bio materials [Category] COVID19(2023년), SARS, 신약개발, [키워드] activity Anti-SARS-CoV-2 Activity Antiviral antiviral activity cell line Combination concentrations confocal microscopy COVID-19 COVID-19 case Cytometry death delivery develop Disease progression drug Drug repurposing encapsulation exhibit fingolimod Flow FTY720 less Lysosomes macropinocytosis nanoparticle Nanotechnology. occur pathway PLGA PLGA-based nanoparticles reduce Safe System therapy for COVID-19 Toxicity Treatment Vaccine viral infection Viral transmission [DOI] 10.1021/acsabm.2c00349 PMC 바로가기 [Article Type] Article
Antibody-dependent enhancement (ADE) of SARS-CoV-2 pseudoviral infection requires FcγRIIB and virus-antibody complex with bivalent interactionSARS-CoV-2 슈도바이러스 감염의 항체 의존성 향상(ADE)에는 2가 상호작용을 갖는 FcγRIIB 및 바이러스-항체 복합체가 필요합니다Article Published on 2022-03-242022-09-12 Journal: Communications Biology [Category] MERS, SARS, 변종, 진단, [키워드] ADE ADE activity B cells bind blocking complex Critical effective ENhance FcγRIIb in vitro Infection inhibit Interaction lysosome acidification mAb mAbs macropinocytosis mechanism molecular mechanism monovalent Neutralizing RBD S trimer Safe SARS-CoV-2 SARS-CoV-2 pseudovirus Therapies understanding while X-ray [DOI] 10.1038/s42003-022-03207-0 PMC 바로가기 [Article Type] Article
Porcine Deltacoronavirus Enters Porcine IPI-2I Intestinal Epithelial Cells via Macropinocytosis and Clathrin-Mediated Endocytosis Dependent on pH and DynaminArticle Published on 2021-09-292023-06-16 Journal: Journal of Virology [Category] MERS, [키워드] caveolae clathrin endocytosis macropinocytosis Porcine deltacoronavirus [DOI] 10.1128/JVI.01345-21 PMC 바로가기
Identifying potential drug targets and candidate drugs for COVID-19: biological networks and structural modeling approachesCOVID-19에 대한 잠재적 약물 표적 및 후보 약물 식별: 생물학적 네트워크 및 구조 모델링 접근법Research Article Published on 2021-05-172022-08-31 Journal: F1000Research [Category] MERS, 치료기술, 치료제, [키워드] acute respiratory syndrome addition Algorithm Analysis Antigen antigen processing approach approach BCL3 binding affinity Biological network analysis Biomarker Biomarkers candidate drug candidate drugs CBL CEBPB complex conducted coronavirus CoV CoV infection COVID-19 CRKL cytokine demonstrated docking result drug target drug targets ERBB4 expressed expression expression profile expression profiles facilitate flexibility growth factor growth factor receptor binding HDAC9 host cell host gene host immune system human pathogen identifying immune signaling pathway include indicated inhibit innate immune response interleukin interleukin-1 KLHL12 Limma macropinocytosis MAPK MAPK signaling medicated MHC class I modulation module molecular dynamics Molecular dynamics simulation molecular dynamics simulations mortality rate MYC NCOA3 network analysis pathway Pathways peptide PPI PPI network protein-protein docking protein-protein interaction receptor Receptor binding receptor signaling reduce regulate Replication respiratory Respiratory system retrieved SARS-CoV-2 SARS-CoV-2 pathogenesis selected Severe acute respiratory syndrome Signal transduction signal transduction pathways Signaling signaling pathways Smad3 SMURF1 stability stimulatory T cell T cell receptor tyrosine VEGFA Walktrap algorithm was selected wortmannin [DOI] 10.12688/f1000research.50850.3 PMC 바로가기 [Article Type] Research Article
A Simplified Quantitative Real-Time PCR Assay for Monitoring SARS-CoV-2 Growth in Cell CultureObservation Published on 2020-09-022022-10-30 Journal: mSphere [Category] COVID-19, SARS, [키워드] activity acute respiratory syndrome addition Alter Alternative pathway antivirals approach assay development bypasse cathepsin B Cell culture cell culture supernatant cell culture supernatants Compound Conventional coronavirus coronavirus disease costly COVID-19 CRISPR cytotoxicity DNA viruses drug Drug screening effective endosomal protease endosomal trafficking etiological agent growth HIV-1 identify in vitro inhibited inhibitor inhibitory concentration macropinocytosis mechanisms MONITOR monitoring Mortality much higher pandemic PCR platform predicted Protease inhibitor quantitative real-time PCR Remdesivir Replication required Research Respiratory disease RNA RNA extraction SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 replication SARS-CoV-2 research SARS-CoV-2 RNA screened severe respiratory disease supernatants Taking time-consuming transcriptase viral entry viral RNA extraction virus replication [DOI] 10.1128/mSphere.00658-20 PMC 바로가기 [Article Type] Observation
A Genome-Wide Haploid Genetic Screen Identifies Heparan Sulfate-Associated Genes and the Macropinocytosis Modulator TMED10 as Factors Supporting Vaccinia Virus InfectionArticle Published on 2019-06-142023-06-10 Journal: Journal of Virology [Category] Fulltext, 원숭이두창, [키워드] genome-wide screen heparan sulfate macropinocytosis Phosphatidylserine poxvirus TMED10 vaccinia virus. [DOI] 10.1128/JVI.02160-18 PMC 바로가기